Cloning of the nucleic acid-binding domain of the rat HnRNP C-type protein |
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| Authors: | Z D Sharp K P Smith Z D Cao S Helsel |
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| Institution: | Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284-78284. |
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| Abstract: | A cDNA encoding the nucleic acid-binding domain of the hnRNP C-type protein has been cloned by DNA-affinity screening of pituitary-derived expression libraries. An analysis revealed sequence identity with the human C-type cDNA and demonstrated the presence of a peptide sequence contained within the single-stranded DNA-binding protein, UP2, which was absent from the human cDNA. Structural analysis of the protein encoded by the rat cDNA demonstrated a net charge of +15 with 14.56% and 6.33% lysines and arginines, respectively, and an amino acid sequence that is consistent with an extensive helix-loop-helix-turn-helix structure. |
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