Determination of derivatized l-alanosine in plasma by liquid chromatography-tandem mass spectrometry |
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Authors: | Gantverg Alex Elliott Gary Pineault Jean Demers Roger |
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Institution: | Maxxam Analytics Inc., 5540 McAdam Road, Mississauga, Ont., Canada L4Z 1P1. alex.gantverg@maxxamanalytics.com |
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Abstract: | A sensitive method was developed for quantitation of the cytotoxic antibiotic l-alanosine in human plasma. Alanosine was extracted from plasma by anion-exchange solid phase extraction, derivatized with dansyl chloride and analyzed by liquid chromatography-tandem mass spectrometry using atmospheric pressure chemical ionization in negative mode. Dansylation led to 50-fold improvement of method sensitivity over non-dansylated alanosine with a resulting 20 ng/ml limit of alanosine quantitation in plasma being achieved. The method was validated and applied for clinical studies of alanosine administered to cancer patients. |
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