Two dimensional electrophoresis of the exo-proteome produced from community acquired methicillin resistant Staphylococcus aureus belonging to clonal complex 80 |
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| Authors: | Shymaa Enany Yutaka Yoshida Sameh Magdeldin Xu Bo Ying Zhang Mohamed Enany Tadashi Yamamoto |
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| Institution: | 1. Department of Structural Pathology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan;2. Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt;3. Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt;4. Department of Bacteriology and Immunology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt |
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| Abstract: | Two-dimensional electrophoresis (2DE) combined with mass spectrometry was used to characterize the exo-proteome secreted by two strains (ER13 and ER21) representing community acquired methicillin resistant Staphylococcus aureus (CA-MRSA) belonging to clonal complex 80 (CC80). Common spots were detected between the 2 gels using the Progenesis SameSpots software. Two hundred and fifty-one and 312 spots from the exo-proteome of ER13 and ER21 were resolved, respectively. 2DE overlap comparison showed that 59 spots were shared. LC–MS/MS analysis identified 57 proteins from these spots comprising about 21% extracellular, 48% cytoplasmic, 2% cytoplasmic membrane, 2% cell wall, and 26% with unknown localization. The identified proteins were classified with respect to their Gene Ontology (GO) annotation as ～24% virulence determinants and toxins, ～17% involved in carbohydrate metabolism, ～14% involved in environmental stress, and ～12% associated with cell division. The identification of the enterotoxin B from the exo-products of both strains used in our study, as belonging to CC80 was interesting. |
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| Keywords: | CA-MRSA Exo-proteome 2DE LC–MS/MS |
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