Expression of creatine kinase isoenzyme during oogenesis and embryogenesis in the mouse |
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Authors: | Melkote R Iyengar Chung Wha L Iyengar Howard Y Chen Ralph L Brinster Elayne Bornslaeger Richard M Schultz |
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Institution: | 1. Department of Animal Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 USA;2. Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 USA |
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Abstract: | Creatine kinase activity was discovered in the growing mouse oocyte and in the preimplantation embryo. Changes in the enzyme activity during the growth and maturation of the egg and during the development of the embryo up to the blastocyst stage were determined. Close similarity of the protein to the brain-type isoenzyme of creatine kinase was established immunochemically. The kinetic parameters of the brain-type isoenzyme (M. R. Iyengar, C. E. Fluellen, and C. W. L. Iyengar, 1982, J. Muscle Cell Motil. 3, 231–246) and the pattern of development-associated changes in activity suggest a possible role for creatine kinase in maintaining the reported high ATP/ADP ratio (L. Ginsberg and N. Hillman, 1975, J. Reprod. Fertil. 43, 83–90), which is essential for the biosynthetic activities of the embryo. |
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Keywords: | To whom all correspondence should be addressed: Department of Animal Biology School of Veterinary Medicine University of Pennsylvania Philadelphia Pa 19104 |
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