Plant regeneration from excised hypocotyl explants of <Emphasis Type="Italic">Platanus acerifolia</Emphasis> willd |
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Authors: | Guofeng?Liu Jun?Huang Longqing?Chen Email author" target="_blank">Manzhu?BaoEmail author |
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Institution: | (1) State Key Laboratory of Crop Genetic Improvement, College of Horticulture and Forestry Science, Huazhong Agricultural University, 430070 Wuhan, P. R. China;(2) Present address: Division of Agriculture, Wuhan Science and Technology Bureau, 430014 Wuhan, P. R. China |
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Abstract: | Summary A method of plant regeneration from hypocotyl segments of Platanus acerifolia Willd, has been developed. Hypocotyl slices were cultured on Murashige and Skoog (MS) basal medium supplemented with a range
of combinations of cytokinins 6-benzyladenine (BA) or kinetin] and auxins indole-3-butyric acid (IBA), indole-3-acetic acid,
α-naphthaleneacetic acid or 2,4-dichlorophenoxyacetic acid] for adventitious shoot induetion. The highest regeneration frequency
was obtained with MS medium containing 2.0 mg l−1 (8.88 μM) BA and 0.5 mg l−1 (2.46 μM) IBA. Adventitious buds and shoots were differentiated from hypocotyl-derived cellus or directly from the wounded sites within
4–8 wk. The regenerated shoots were elongated and proliferated efficiently on multiplication medium. Complete plantlets were
transplanted to the soil and grew normally in the greenhouse after root formation on rooting medium for 4–6 wk. |
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Keywords: | Platanus acerifolia hypocotyl shoot regeneration organogenesis tissue culture |
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