茉莉酸甲酯对木豆不定根染料木素含量及抗氧化系统影响

为探讨茉莉酸甲酯(Methyl Jasmonate,MJ)对木豆不定根影响,研究了茉莉酸甲酯的不同浓度及处理时间对木豆不定根中染料木素的含量及抗氧化系统的影响。研究结果表明,诱导子的浓度和处理时间对不定根中染料木素的含量有重要影响。在MJ浓度为100μmol·L^-1,处理时间为48 h时,染料木素的含量最高,为1.38 mg·g^-1,是对照组的4.03倍。在此基础上,研究了100μmol·L^-1的MJ处理对木豆不定根抗氧化系统的影响,结果表明,MJ处理的木豆不定根明显发生了氧化胁迫,O_2^-速率、H_2O_2和MDA含量明显升高,同时不定根中SOD和POD活性明显升高,但是抗氧化酶活性的提高并未消除不定根的氧化胁迫,这种氧化胁迫导致了木豆不定根次生代谢产物合成能力提高。

Effects of Methyl Jasmonate on Genistein Content and Antioxidant System of Adventitious Roots of Cajanus cajan(L.) Millsp.

We studied the effect of different concentrations and treatment time of Methyl jasmonate(MJ) ongenistein production in adventitious roots. The concentrations and treatment time of MJ had a significant effect on the content of genistein in pigeon pea adventitious roots. When the concentration of MJ was 100 μmol·L^-1, and the treatment time was 48 h, the content of genistein was the highest(1.38 mg·g^-1), which was 4.03 times higher than that of the control. We studied the effect of MJ(100 μmol·L^-1) on the antioxidant system inpigeon pea adventitious roots.The adventitious roots treated with MJ appeared obvious oxidative stress, the contents of O₂^-, H₂O₂ and MDA increased significantly, and the activities of SOD and POD also increased significantly. However, the increase of antioxidant enzyme activity couldnot eliminate the oxidative stress inpigeon peaadventitious roots, leding to the improvement of secondary metabolites synthesis.