鉴定免疫细胞中DNA疫苗结合蛋白

Succinate Production from Escherichia coil Mutant QQS101 Fermentation

Succinate is an important bio-based platform molecule. In the present work, the growth and glucose metabolism of Escherichia coli mutant QQS101 with the deficiency of formate transporter A (focA), formate-pyruvate lyase (pflB) and lactate dehydrogenase (ldhA) under strict anaerobic condition were investigated. The degree of reduction per carbon of glucose and the products of E. coli mix-acids fermentation were compared, and then recognized that non-strict anaerobic condition favored QQS101 producing succinate from glucose. Furthermore, effects of the carbon sources for aerobic growth on fermentation were performed. Results showed that QQS101 could accumulate succinate with a concentration of 31.01 g/L with a yield of 1.258 mol Succinate/mol Glucose, when growing on xylose as the aerobic substrate. During the fermentation, addition of alanine could enhance the molar yield of succinate to glucose utilized.