The use of genomic in situ hybridization (GISH) to show transmission of recombinant chromosomes by a partially fertile bigeneric hybrid, Gasteria lutzii ×Aloe aristata (Aloaceae), to its progeny |
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Authors: | C Takahashi I J Leitch A Ryan M D Bennett P E Brandham |
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Institution: | (1) Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, UK, GB |
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Abstract: | Genomic in situ hybridization (GISH) was used to study somatic chromosomes of parental and progeny plants (all 2n=2x=14) of the bigeneric hybrid between Gasteria lutzii and Aloe aristata (Aloaceae), which is partially fertile, a rare occurrence in plants. GISH successfully distinguished between the two parental
genomes in the F1 hybrid and revealed numerous genomic recombinations in chromosomes transmitted by the F1 to the back-cross
progeny. The results indicate high levels of meiotic compatibility between the parental genomes, even though they differ in
size by 20%. Recombination occurred at a frequency that was higher than that expected from the analysis of orcein-stained
meiosis in the F1. The discrepancy suggests that terminalization may occur prior to or during metaphase I, reducing the apparent
chiasma frequency, or possibly reveals an under-estimation caused by difficulties in resolving closely grouped chiasmata by
eye.
Received: 9 September 1996; in revised form: 21 October 1996 / Accepted: 21 October 1996 |
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