Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061 |
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| Authors: | Chun-Gyu Kim Janardan Lamichhane Kwang-Il Song Van D. Nguyen Dong-Hee Kim Tae-Sung Jeong Sung-Ho Kang Kyoung-Wook Kim Jyoti Maharjan Young-Soo Hong Jae Seon Kang Jin-Cheol Yoo Jung-Joon Lee Tae-Jin Oh Kwangkyoung Liou Jae Kyung Sohng |
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| Affiliation: | (1) Department of Pharmaceutical Engineering, Inje University, Kimhae, Kyung-nam, 621-749, Republic of Korea;(2) Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, Sun Moon University, #100, Kalsan-ri, Tangjeong-myeon, Asansi, Chungnam, 336-708, Republic of Korea;(3) Korea Research Institute of Bioscience and Biotechnology, KIST, Daejeon, 305-600, Republic of Korea;(4) Department of Pharmacy, Chosun University, Kwangju, 501-759, Republic of Korea;(5) Department of Pharmaceutical Engineering, Sun Moon University, #100, Kalsan-ri, Tangjeong-myeon, Asansi, Chungnam, 336-708, Republic of Korea |
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| Abstract: | The four overlapping cosmids from the rubradirin producer, Streptomyces achromogenes var rubradiris NRRL 3061, have 58 ORFs within a 105.6 kb fragment. These ORFs harbored essential genes responsible for the formation and attachment of four distinct moieties, along with the genes associated with regulatory, resistance, and transport functions. The PKS (rubA) and glycosyltransferase (rubG2) genes were disrupted in order to demonstrate a complete elimination of rubradirin production. The rubradirin biosynthetic pathway was proposed based on the putative functions of the gene products, the functional identification of sugar genes, and the mutant strains. The GeneBank accession number for the sequence reported in this paper is AJ871581. |
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| Keywords: | Ansamycin Biosynthesis Polyketide synthase Rubradirin Rubranitrose Streptomyces |
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