碱性成纤维细胞生长因子增加大鼠血管一氧化氮及内皮素的生成

研究碱性成纤维细胞生长因子（ｂａｓｉｃ ｆｉｂｒｏｂｌａｓｔ ｇｒｏｗｔｈ ｆａｃｔｏｒ,ｂＦＧＦ）对自发性高血压大鼠（ＳＨＲ）和ＷＫＹ大鼠血管一氧化氮（ＮＯ）及内皮素生成的影响。取ＳＨＲ和ＷＫＹ大鼠主动脉制成血管薄片,以１０、１００ｎｇ／ｍｌ ｂＦＧＦ（终浓度）分别孵育６ｈ,测定血管组织中一氧化氮合酶（ＮＯＳ）活性及孵育液中亚哨酸盐（ＮＯ２）和内皮素含量。结果显示：ＳＨＲ主动脉组织ＮＯＳ活性较Ｗ

Basic fibroblast growth factor increases nitric oxide and endothelin production in rat aorta

The study was undertaken to investigate the effect of basic fibroblast growth factor (bFGF) on aortic production of nitric oxide (NO) and endothelin of aorta in spontaneously hypertensive rats (SHR) and WKY rats. Rat aortas were cut into vessel slices and incubated with 10 or 100 ng/ml bFGF for 6 hours. Nitric oxide synthase (NOS) activity in aortic slices and contents of NO 2 and endothelin in the medium were de~ter~mined. The results showed that NOS activity in aortic slices of SHR was 17 6% lower than that of WKY ( P <0 01). NO 2 and endothelin contents in the medium of SHR aortic slices were 59 7% lower and 37 4% higher than those of WKY aortic slices. Upon the exposure of low and high doses of bFGF, NOS activity in the aorta of SHR was increased by 29 7% and 59 6% (both P<0 01),respectively, while the NO 2 contents in the medium were increased respectively by 28 2% (P<0 05) and 70 5% (P<0 01). Aortic endothelin production was increased by 24 1% and 44 5%(both P<0 01) respectively while the NOS activity in the aorta of WKY was increased by 24 4% and 53 7% (both P<0 01). NO 2 contents in the medium were augmented by 18 8% (P<0 05)and 25 9% (P<0 01), respectively. Aortic endothelin production was increased by 84 1% and 93 1% (both P<0 01). It is concluded that bFGF may modulate the production of NO and endothelin in both SHR and WKY rats.