去除Wolbachia 对丽蝇蛹集金小蜂繁殖适合度和成蜂寿命的影响

【目的】明确内共生菌 Wolbachia 对丽蝇蛹集金小蜂 Nasonia vitripennis 繁殖适合度和成蜂寿命的影响。【方法】通过给自然感染 Wolbachia 的丽蝇蛹集金小蜂成蜂喂食不同浓度的利福平来消除其体内的 Wolbachia，然后进行10个世代的连续饲养，探究不同浓度利福平对丽蝇蛹集金小蜂体内 Wolbachia 的去除效果和去除 Wolbachia 后对丽蝇蛹集金小蜂繁殖力、性比（雌蜂占子代数量的比值）和成蜂寿命的影响。【结果】低浓度利福平（0.1~0.5 mg/mL）对丽蝇蛹集金小蜂的毒害作用较小，而高浓度利福平（0.7~10.0 mg/mL）对丽蝇蛹集金小蜂的毒害作用较大,但二者均能去除丽蝇蛹集金小蜂体内的 Wolbachia；去除 Wolbachia 后丽蝇蛹集金小蜂的出蜂量显著下降（P <0.01），子代中性比显著下降（P <0.01），但寿命无明显差异。【结论】不同浓度利福平均能去除丽蝇蛹集金小蜂体内Wolbachia，但效果不一致；Wolbachia 对丽蝇蛹集金小蜂的出蜂量和子代性比均有显著影响，对成蜂寿命无显著影响。

Influence of removing Wolbachia on the reproductive fitness and adult longevity of Nasonia vitripennis (Hymenoptera: Pteromalidae)

【Aim】 This study aims to clarify the influence of endosymbiont Wolbachia on the reproductive fitness and adult longevity of Nasonia vitripennis. 【Methods】 Different concentrations of rifampicin (a kind of antibiotics) were used to feed N. vitripennis adults for removing Wolbachia in their bodies to assess the effect of Wolbachia on the reproductive fitness and adult longevity of N.vitripennis in the next ten consecutive generations. 【Results】 The results showed that the low rifampicin concentrations (0.1-0.5 mg/mL) had lower toxicity, while high rifampicin concentrations (0.7-10.0 mg/mL) had higher toxicity to N. vitripennis, but Wolbachia could be removed by all the concentrations of rifampicin. The number of offspring as well as sex ratio (female to all offspring ratio) of N. vitripennis decreased significantly after removing Wolbachia, while there was no significant difference in adult longevity between the Wolbachia infected and uninfected individuals. 【Conclusion】 All the different concentrations of rifampicin can remove Wolbachia from N. vitripennis, but the efficiency varies. Wolbachia has distinct effects on the number of offspring and sex ratio of N. vitripennis, while has no evident influence on its adult longevity.