Production of Two Vaccinating Recombinant Rotavirus Proteins in the Milk of Transgenic Rabbits |
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Authors: | Eric Soler Agnès Le Saux Frédéric Guinut Bruno Passet Ruxandra Cohen Christine Merle Annie Charpilienne Cynthia Fourgeux Véronique Sorel Antoine Piriou Isabelle Schwartz-Cornil Jean Cohen Louis-Marie Houdebine |
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Institution: | (1) Biologie du Développement et de la Reproduction, INRA, bat.440, 78352 Jouy-en-Josas, France;(2) Bioprotein Technologies, 63–65 bd Masséna, Paris, France;(3) Virologie Moléculaire & Structurale, CNRS-INRA, Gif-sur-Yvette, France;(4) Virologie et Immunologie Moléculaires, INRA, Jouy-en-Josas, France |
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Abstract: | Rotaviruses are the main cause of infantile viral gastroenteritis worldwide leading to approximately 500,000 deaths each year
mostly in the developing world. For unknown reasons, live attenuated viruses used in classical vaccine strategies were shown
to be responsible for intussusception (a bowel obstruction). New strategies allowing production of safe recombinant non-replicating
rotavirus candidate vaccine are thus clearly needed. In this study we utilized transgenic rabbit milk as a source of rotavirus
antigens. Individual transgenic rabbit lines were able to produce several hundreds of micrograms per ml of secreted recombinant
VP2 and VP6 proteins in their milk. Viral proteins expressed in our model were immunogenic and were shown to induce a significant
reduction in viral antigen shedding after challenge with virulent rotavirus in the adult mouse model. To our knowledge, this
is the first report of transgenic mammal bioreactors allowing the rapid co-production of two recombinant viral proteins in
milk to be used as a vaccine. |
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Keywords: | bioreactor milk rotavirus VP2 VP6 |
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