Aberrant T cell activation and heightened apoptotic turnover in end-stage renal failure patients: a comparative evaluation between non-dialysis,haemodialysis, and peritoneal dialysis |
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Authors: | Moser Bernhard Roth Georg Brunner Markus Lilaj Tatjana Deicher Robert Wolner Ernst Kovarik Josef Boltz-Nitulescu George Vychytil Andreas Ankersmit Hendrik Jan |
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Institution: | Department of Surgery, General Hospital of Vienna, Austria. |
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Abstract: | Patients in end-stage renal disease (ESRD) have a high incidence of bacterial and viral infections. Fifteen non-dialysed (ND), 15 haemodialysed (HD), 15 patients with peritoneal dialysis (PD), and 15 healthy controls were included. T cell proliferation was measured by 3H]thymidine uptake. Apoptosis and cell phenotype were determined by FACS. sTNF-R1, sCD95, interleukin-1beta-converting enzyme (sICE), and interleukin (IL)-10 were measured by ELISA. PHA and CD3-driven T cell proliferation were significantly decreased in ESRD patients. CD3(+), CD19(+) B cells, and percentage of CD4(+) T cells were significantly reduced. Percent memory T cells (CD45RO(+)) and cells undergoing apoptosis (CD95(+)/Annexin V+) were significantly increased in ESRF. Moreover, sCD95, sTNFRI, and ICE were significantly increased. Serum level of IL-10, a Th2 cytokine, was enhanced. These findings strongly suggest that in ESRD patients Th1 T cells are selectively susceptible to undergo apoptosis. This observation provides an additional pathophysiological concept in the genesis of Th2 dominance. |
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Keywords: | Apoptosis Chronic renal failure Haemodialysis Peritoneal dialysis T cell activation Caspase CD95 Cell death Dialysis ESRD |
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