Amino acid production from rice straw and wheat bran hydrolysates by recombinant pentose-utilizing <Emphasis Type="Italic">Corynebacterium glutamicum</Emphasis> |
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Authors: | Vipin Gopinath Tobias M Meiswinkel Volker F Wendisch K Madhavan Nampoothiri |
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Institution: | (1) Biotechnology Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Trivandrum, 695 019, Kerala, India;(2) Chair of Genetics of Prokaryotes, Faculty of Biology & CeBiTec, Bielefeld University, Universitaetsstr. 25, 33615 Bielefeld, Germany; |
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Abstract: | Corynebacterium glutamicum wild type lacks the ability to utilize the pentose fractions of lignocellulosic hydrolysates, but it is known that recombinants
expressing the araBAD operon and/or the xylA gene from Escherichia coli are able to grow with the pentoses xylose and arabinose as sole carbon sources. Recombinant pentose-utilizing strains derived
from C. glutamicum wild type or from the l-lysine-producing C. glutamicum strain DM1729 utilized arabinose and/or xylose when these were added as pure chemicals to glucose-based minimal medium or
when they were present in acid hydrolysates of rice straw or wheat bran. The recombinants grew to higher biomass concentrations
and produced more l-glutamate and l-lysine, respectively, than the empty vector control strains, which utilized the glucose fraction. Typically, arabinose and
xylose were co-utilized by the recombinant strains along with glucose either when acid rice straw and wheat bran hydrolysates
were used or when blends of pure arabinose, xylose, and glucose were used. With acid hydrolysates growth, amino acid production
and sugar consumption were delayed and slower as compared to media with blends of pure arabinose, xylose, and glucose. The
ethambutol-triggered production of up to 93 ± 4 mM l-glutamate by the wild type-derived pentose-utilizing recombinant and the production of up to 42 ± 2 mM l-lysine by the recombinant pentose-utilizing lysine producer on media containing acid rice straw or wheat bran hydrolysate
as carbon and energy source revealed that acid hydrolysates of agricultural waste materials may provide an alternative feedstock
for large-scale amino acid production. |
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