Synaptotagmin-7 controls the size of the reserve and resting pools of synaptic vesicles in hippocampal neurons |
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| Institution: | 1. Dpto. Fisiología Médica y Biofísica, Facultad de Medicina, Universidad de Sevilla, 41009 Sevilla, Spain;2. Research Group Molecular Mechanisms of Synaptic Function, Max Planck Florida Institute, Jupiter, FL 33458. USA;1. Departments of Cell Biology and Physiology, Biomedical Engineering, Washington University, St. Louis, MO 63110, USA;1. Department of Biology, Virginia Commonwealth University, Richmond, VA 23284-2012, United States;2. Department of Forensic Science, Virginia Commonwealth University, Richmond, VA 23284-2012, United States;3. Department of Biology, Manhattan College, Riverdale, NY 10471, United States;1. Department of Molecular and Cellular Physiology and Howard Hughes Medical Institute, Stanford University Medical School, Stanford, CA 94305, USA;2. Nancy Pritzker Laboratory, Department of Psychiatry and Behavioral Sciences, Stanford University Medical School, Stanford, CA 94305, USA;1. Truhlsen Eye Institute and Department of Ophthalmology and Visual Sciences, USA;2. Pharmacology and Experimental Neuroscience, USA;3. Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, College of Medicine, University of Nebraska Medical Center, Omaha, NE, 68106, USA;1. Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany;2. Department of Cellular Biophysics, Institute for Medical Physics and Biophysics, University of Münster, 48149 Münster, Germany;3. Department of Molecular Pharmacology & Cell Biology, Leibniz Institut für Molekulare Pharmakologie, 13125 Berlin, Germany;4. Center Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB), 37073 Göttingen, Germany;5. Cluster of Excellence EXC 1003, Cells in Motion, CiM, 48149 Münster, Germany;1. Max-Planck-Institut for Biophysical Chemistry, 37077 Göttingen, Germany |
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| Abstract: | Continuous neurotransmitter release is subjected to synaptic vesicle availability, which in turn depends on vesicle recycling and the traffic of vesicles between pools. We studied the role of Synaptotagmin-7 (Syt-7) in synaptic vesicle accessibility for release in hippocampal neurons in culture. Synaptic boutons from Syt-7 knockout (KO) mice displayed normal basal secretion with no alteration in the RRP size or the probability of release. However, stronger stimuli revealed an increase in the size of the reserve and resting vesicle pools in Syt-7 KO boutons compared with WT. These data suggest that Syt-7 plays a significant role in the vesicle pool homeostasis and, consequently, in the availability of vesicles for synaptic transmission during strong stimulation, probably, by facilitating advancing synaptic vesicles to the readily releasable pool. |
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| Keywords: | Synapse Vesicle pools Synaptotagmin Exocytosis Synaptic transmission |
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