贵州野生大麦麦芽品质性状的SSR标记分析

采用6对啤酒大麦的麦芽浸提和糖化力紧密连锁引物对103份采自贵州的野生大麦材料进行SSR标记。结果表明，贵州野生大麦麦芽品质性状存在丰富的变异，6对SSR引物共检测出38个等位变异，每个位点平均6.33个等位变异，其中GMS001位点对贵州野生大麦基因组DNA变异检测最有效。UPGMA聚类图显示，该6对与麦芽品质紧密连锁的SSR引物对区分野生大麦在贵州不同的资源产地和棱性是有效的，表现为遵义地区野生大麦遗传多样性丰富，而来自贵州凯里地区的野生大麦资源遗传多样性狭窄。麦芽品质性状标记结果表明贵州野生六棱大麦较四棱大麦的遗传差异更显著，表明进行贵州啤酒大麦人工育种的亲本应在亲缘关系较远的六棱大麦之间选择。

Assessment of Genetic Diversity in Guizhou Wild Barley Using SSR Markers Ralated to Malting Quality

6 SSR markers ralated to traits of diastatic power and enzyme extraction of malting quality were applied to analyze genetic diversity in one hundred and three accessions of wild barley (Hordeum vulgare L.) from Guizhou. As shown in the present study, a total of 38 alleles were detected with an average of 6.33 per SSR primer pair. Our results indicated that locus GMS001 was effectively applied to detect DNA variation among Guizhou wild barleys, based on higher values of polymorphism information content(PIC) as compared with those of Australian barley cultivars. The dendrogram by UPGMA cluster analysis revealed that the selected SSR markers may be applied to separate phenotype and resource classification of 103 materials obtained from Guizhou. Namely, genetic diversity of the wild barley obtained from Zunyi district was abundant, as compared with materials from the other districts. Moreover, more significant genetic difference was observed in six-row wild barley than in -row material. It is suggested that choice of beer barley parents should be performed among populations of six-rowed wild barley in Guizhou, based on their abundant genetic diversity.