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Characteristics of mixotrophic growth of Synechocystis sp. in an enclosed photobioreactor
Authors:Yonghong Wang  Yuanguang Li  Dingji Shi  Guomin Shen  Binggen Ru  Siliang Zhang
Institution:(1) Marine Bioprocess Engineering Institute, State Key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China;(2) Research Center of Photosynthesis, Institute of Botany, The Chinese Academy of Sciences, Beijing, 100093, China;(3) College of Life Science, Peking University, Beijing, 100871, China
Abstract:Synechocystis sp. PCC 6803 was grown in a 2.5 l enclosed photobioreactor on medium with or without glucose. The incident light intensities ranged from 1.5 klux to 7 klux. The highest average specific growth rates of mixotrophic culture and photoautotrophic culture were, respectively, 1.3 h–1 at a light intensity of 7 klux on 3.2 g l–1 glucose and 0.3 h–1 at both light intensities of 5 klux and 7 klux. The highest cell density 2.5 g l –1 was obtained at both of light intensities 5 klux and 7 klux on 3.2 g glucose l–1. Glucose consumption decreased with decreasing light intensity. The energy yields of mixotrophic cultures were 4 to 6 times higher than that of photoautotrophic cultures. Light favored mixotrophic growth of Synechocystis sp. PCC 6803, especially at higher light intensities (5–7 klux).
Keywords:mixotrophic growth  photobioreactor  Synechocystis sp  
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