Localization in situ of type VI collagen protein and its mRNA in mesangial proliferative glomerulonephritis using renal biopsy sections |
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Authors: | M S Razzaque T Koji T Harada T Taguchi |
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Institution: | (1) Second Department of Pathology, Nagasaki University School of Medicine, 1-12-4, Sakamoto, Nagasaki 852, Japan e-mail: razzaque@net.nagasaki-u.ac.jp, JP;(2) Third Department of Anatomy, Nagasaki University School of Medicine, Nagasaki, Japan, JP;(3) Second Department of Medicine, Nagasaki University Hospital, Nagasaki, Japan, JP |
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Abstract: | Extracellular matrix accumulation is crucial in the pathogenesis of glomerulosclerosis in mesangial proliferative glomerulonephritis
(GN). In an attempt to explore the distribution of type VI collagen and its synthesizing cells in normal and diseased glomeruli,
we investigated mRNA and protein expression of type VI collagen in renal biopsy sections, histologically diagnosed as mesangial
proliferative GN. Five renal biopsies from patients diagnosed as having minor glomerular abnormalities and one surgical renal
tissue were also simultaneously examined as controls. Immunohistochemical studies revealed type VI collagen immunostaining
in the mesangium and glomerular basement membrane of the control glomeruli. Compared to the control, increased deposition
of type VI collagen was noted in the mesangial proliferative and sclerotic lesions in GN. To identify the cells responsible
for the synthesis of type VI collagen mRNA, renal sections were hybridized in situ with digoxigenin-labeled antisense oligo-DNA
probe complementary to a part of α1 (VI) mRNA. Occasionally intraglomerular cells hybridized with digoxigenin-labeled antisense
pro α1 (VI) oligo-DNA in control glomeruli. An increased number of intraglomerular cells (mostly epithelial cells) were, however,
positive for α1 (VI) mRNA expression in GN sections. The present study documents the distribution of type VI collagen in the
normal glomeruli and provides further evidence of accelerated synthesis of this collagen in mesangial proliferative GN.
Accepted: 21 July 1998 |
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