渗透剂对白刺花体细胞胚成熟及萌发的影响

该研究以蔗糖、麦芽糖、山梨醇及PEG(6000)为渗透剂,探讨了不同渗透剂对白刺花体细胞胚发育、胚成熟及萌发的影响。结果表明:白刺花下胚轴形成的胚性愈伤组织接种至MS+2,4-D 0.2 mg·L~(-1)+NAA 1.0 mg·L~(-1)+6-BA 2.0 mg·L~(-1)+TDZ 1.0 mg·L~(-1)+蔗糖40 g·L~(-1)+谷氨酰胺100 mg·L~(-1)+植物凝胶3g·L~(-1)的培养基上,体细胞胚发生率高达66. 21%,总胚数为79个; 7%蔗糖可使体细胞胚成熟率高达64.36%,同时也可提高多子叶畸形胚形成; 2%麦芽糖+2%山梨醇+4%蔗糖组合使体细胞胚成熟率最高达88.89%,畸形胚比例最低; 30 g·L~(-1)PEG培养时,体细胞成熟率最高,为82.35%;鱼雷期的体细胞胚最合适转接,可使体胚萌发率达90.58%,复合糖上培养得到的成熟体细胞胚生根率最高,为87.47%。这为实现白刺花体细胞胚育苗奠定了理论基础,并提供了可行的方案。

Effects of penetrant on maturation and germination for somatic embryo of Sophora davidii

To discuss the effect of different penetrants on maturation and germination of somatic embryo of Sophora davidii. Sucrose, maltose, sorbitol and PEG(6000)were used as penetrant in this paper. The development, maturation, and germination of somatic embryo was determined by different penetrant comparation. The results were as follows: the rate of somatic embryogenesis reached up to 66.21%, and the total number of embryos can achieve 79 when embryonic callus from hypocotyl of Sophora davidii were cultured in MS+ 2,4-D 0.2 mg·L^-1 +NAA 1.0 mg·L^-1+ 6-BA 2.0 mg·L^-1 + TDZ 1.0 mg·L^-1+sucrose 40 g·L^-1+ glutamine 100 mg·L^-1+plant gel 3 g·L^-1. Adding 7% sucrose could promote the maturation rate of somatic embryo to 64.36%, meanwhile could also increase the development of polycotyledonous abnormal embryo. The maturation rate of somatic embryo went up as high as 88.89%, and the proportion of abnormal embryo the lowest, when somatic embryo were cultured in 2% maltose +2% sorbitol +4% sucrose combination medium. The maturation rate of somatic embryo was 82.35%, when the concentration of PEG was 30 g·L^-1. Torpedo embryo was suitable for transported culture, the formative rate of somatic embryo was up to 90.58%. The rooting rate of mature somatic embryo was high, for 87.47% from three sugar combination culture. This study provides a theoretical reference and a feasible scheme for the development of somatic embryo seedling of Sophora davidii.