Restriction analysis of the Frankia spp. genome |
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| Authors: | Svetlana V Dobritsa |
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| Institution: | Institute of Biochemistry and Physiology of Microorganisms, U.S.S.R. Academy of Sciences, Pushchino, Moscow Region, 142292, U.S.S.R. |
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| Abstract: | Abstract Total cellular DNAs of 10 Frankia isolates from Alnus, Elaeagnus and Colletia spp. root nodules were cleaved with ten site-specific restriction endonucleases and analysed by agarose gel electrophoresis. Among the endonucleases tested, Bam HI, Bgl II, Sal I and Sma I proved to be the most suitable for the genome analysis in Frankia spp. DNA restriction banding patterns were reproducible and characteristic of each Frankia strain. The patterns of different strains differed marked indicating considerable genotypic heterogeneity among the isolates. The approach can be used for strain identification in Frankia spp. as well as for differentiation between phenotypically similar strains. |
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| Keywords: | Total DNA restriction endonuclease DNA fragment banding pattern genome heterogeneity |
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