| 人生长激素基因的cDNA克隆,原核高效表达及纯化 |
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| 引用本文： | 葛永红,何黔川.人生长激素基因的cDNA克隆,原核高效表达及纯化[J].微生物学免疫学进展,2000,28(3):11-14. |
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| 作者姓名： | 葛永红 何黔川 |
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| 作者单位： | 成都生物制品研究所!610063 |
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| 摘 要： | 用ＲＴ－ＰＣＲ方法，从含有全长人生长激素（ＨＧＨ）基因的ＣＨＯ细胞中获得ｈｇｈ的ｃＤＮＡ，将其克隆入ｐＥＴ－１１ｂ载体中，在大肠杆菌中获得高效表达，表达量占菌体蛋白总量的２０％。经色谱纯化后，纯度大于９５％。
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| 关 键 词： | 重组人生长激素 原核表达 纯化 基因克隆 |
Cloning,high prokaryotic expression and purification of human growth hormone |
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| GE Yong hong,HE Qian chuan,LI Juan,et al..Cloning,high prokaryotic expression and purification of human growth hormone[J].Progress In Microbiology and Immunology,2000,28(3):11-14. |
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| Authors: | GE Yong hong HE Qian chuan LI Juan |
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| Abstract: | By the method of RT PCR,the cDNA of human growth hormone(HGH)was amplified from the CHO cells transfected with the full length DNA of HGH gene.The cDNA was cloned into the vector pET 11b followed by transforming the E.coli BL21(DE3).The recombinant HGH was expressed by the induction of IPTG at a ratio of 20% of total proteins.After chromatograph,the purity of rHGH is higher than 95%. |
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| Keywords: | Recombinant HGH Cloning Prokaryotic expression Purification |
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