Effects of chemical and physical mutagens on the frequency of a large genetic duplication in Salmonella typhimurium. II. Stimulation of duplication-loss from merodiploids. |
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Authors: | G R Hoffmann R W Morgan R Kirven |
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Institution: | 1. National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA;2. Department of Biology, Meredith College, Raleigh, NC 27611, U.S.A. |
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Abstract: | Strains of Salmonella typhimurium which contain a duplication of approximately 30% of the genome may be obtained by a simple selective procedure. These strains are highly unstable, losing the duplication when grown on non-selective medium. In this paper we report that treatment of merodiploid bacteria with mutagenic agents stimulates the rate at which haploid segregants are obtained from merodiploid strains. The mutagens which have been tested for this effect are X-rays, ultraviolet light (UV), ethyl methanesulfonate (EMS), and the azaacridine half-mustard ICR-372. |
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Keywords: | EMS ethyl methanesulfonate malate fast grower (contains genetic duplication) |
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