胞内钙平衡在培养的牛主动脉内皮细胞低氧和复氧损伤中的作用

本文通过建立培养的牛主动脉内皮细胞体外低氧复氧模型，观察低氧和复氧时胞内钙浓度的改变与存活率的关系。结果显示，随低氧时间延长内皮细胞存活率下降，低氧后再复氧存活率进一步降低。低氧时无钙溶液孵育降低细胞的存活率，但复氧时无钙溶液孵育则增加细胞的存活率。低氧２ｈ胞内钙浓度从９９ｎｍｏｌ／Ｌ降对６９ｎｍｏｌ／Ｌ，无钙时胞内钙进一步降低，低氧４ｈ再复氧４０ｍｉｎ，胞内钙浓度恢复至正常。提示细胞内钙浓度平衡

ROLE OF CYTOSOLIC CALCIUM BALANCE ON CELL INJURY IN CULTURED BOVINE AORTIC ENDOTHELIAL CELLS DURING HYPOXIA AND REOXYGENATION

Making use of an in vitro model of bovine aortic endothelial cell monolayers, thecell viability and changes of intracellukar calcium concentration of the preparation weresubmitted to hypoxia with or without reoxygenation. It was shown that the cell viabilitydeclined under hypoxia but increased under reoxygenation when the endothelial cellswere incubated in calcium-free HBSS, as compared with control HBSS incubation. Itwas observed that a significant reduction of intracellular calcium from 99 to 69 nmol/L during 2 h of hypoxia, which was further decreased in Ca2 -free HBSS medium. After 4h hypoxia followed by 40 min reoxygenation, Ca2 ]i, recovered to the nomal concentration. It is speculated that the calcium homeostasis may be an important conditionfor cells to carry out normal function and either decrease or increase of Ca2 ]i may injury bovine aortic endothelial cells.