Semi-automated solid-phase DNA sequencing. |
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| Authors: | M Uhlén T Hultman J Wahlberg J Lundeberg S Bergh B Pettersson A Holmberg S St?hl T Moks |
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| Affiliation: | Department of Biochemistry and Biotechnology, Royal Institute of Technology, Stockholm, Sweden. |
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| Abstract: | Increasing the efficiency of DNA sequencing necessitates the development of systems which reduce the need for manual operations by integrating template preparation, sequencing reactions, product separation and detection. A semi-automated system, whereby PCR-amplified biotinylated genomic or plasmid DNA is immobilized on streptavidin-coated magnetic beads, has been developed. |
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