Detection of phospholipid peroxides in biological samples |
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Authors: | Frederik JGM van Kuijk Edward A Dratz |
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Institution: | Department of Chemistry, Montana State University, Bozeman 59717. |
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Abstract: | Peroxidation of membrane lipids has been hypothesized to play a key role in various types of tissue degeneration and pathology. Lipid peroxides are formed when oxygen reacts with an unsaturated fatty acid chain. Virtually all of the unsaturated fatty acids in biological systems are bound by ester linkages in phospholipids or triglycerides. Phospholipid and triglyceride peroxides are primary products of lipid peroxidation and have rarely been measured. Most of the commonly used methods for detection of lipid peroxidation are based on detection of malondialdehyde or other chemical species that are derived from oxidized fatty acids. This review presents an overview of recently developed methods aimed at identifying and measuring oxidized phospholipids and triglycerides which are direct evidence of the occurrence of lipid peroxidation in vivo. |
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Keywords: | Lipid peroxidation Lipid autoxidation Phospholipid peroxides Colorimetric assay Enzymatic assay Ultraviolet absorption spectra High performance liquid chromatography Gas chromatography-mass spectrometry |
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