<Emphasis Type="SmallCaps">l</Emphasis>(+)-Lactic acid production by co-fermentation of glucose and xylose with <Emphasis Type="Italic">Rhizopus oryzae</Emphasis> obtained by low-energy ion beam irradiation |
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Authors: | Peng Wang Juan Li Li Wang Ming-li Tang Zeng-liang Yu Zhi-ming Zheng |
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Institution: | (1) Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, 1126, 230031 Hefei, Anhui, People’s Republic of China |
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Abstract: | Low-energy ion beam irradiation (10–200 keV) has been proved to have a wide range of biological effects in recent years. When
Rhizopus oryzae PW352 was irradiated with a 15-keV low-energy ion beam an l(+)-lactic acid high-yield mutant, RQ4015, was obtained. When 150 g/l glucose was used as the sole carbon source, l(+)-lactic acid of RQ4015 reached 121 g/l after 36 h shake-flask cultivation. However, the highest lactic acid concentration
74 g/l was obtained when 100 g/l xylose was present in the medium as the sole carbon source. When mixed xylose (25 g/l) and
glucose (75 g/l) were present in a bubble column, l(+)-lactic acid production of RQ4015 reached 83 g. A high mutation rate and a wide mutation spectrum of low-energy ion implantation
were observed in the experiment, suggesting that ion implantation can be a highly efficient mutagenic means for microorganism
breeding in many commercial applications. |
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