38份晾晒烟种质资源遗传关系的SSR分析

利用SSR标记技术对38份晾晒烟种质资源的遗传关系进行了分析。从自己开发的近3000对烟草SSR引物中随机选出30对引物，在38份供试材料中共检测出173个等位基因，每对SSR引物可检测的等位基因数为2~11个，平均为5.77个。38份材料间遗传相似系数（GS）的变化范围为0.165-0.928，平均GS 为0.546。表明38份晾晒烟的遗传多样性丰富，遗传差异较大，亲缘关系较远。聚类分析表明，在L1（GS-0.165）处可将38个品种分为2大类，即晾晒烟类群和美国从烟草起源地收集的烟草（TI：Tobacco Instruction）类群；晾晒烟类群又可进一步分为4组，其聚类结果与所期望的结果基本一致。表明SSR是一种有效、稳定和可靠的分子标记，能较好地从分子水平上揭示烟草(尤其是晾晒烟)种质资源的遗传背景和亲缘关系。

Genetic Relationship Analysis of Thirty-eight Sun/Air-Cured Tobacco Germplasms Based on Simple Sequence Repeat (SSR) Markers

We report the first phylogenesis of 38 Sun/Air-Cured tobacco germplasms analyzed by Simple Sequence Repeat (SSR) Markers in this study. 30 primer pairs were randomly selected from a nearly total of 3000 tobacco SSR primers which were developed by our own. A total of 173 polymorphic alleles were amplified in 38 varieties using 30 SSR primers, and the number of alleles detected by each SSR primer ranged from 2 to 11, with an average of 5.77 alleles per markers. Genetic similarity coefficient (GS) among the 38 varieties ranged from 0.165 to 0.928, with an average of 0.546, which indicated the genetic variation of the 38 varieties was quite large and the genetic diversity was much abundant in Sun/Air-Cured tobacco varieties. The result of cluster analysis based on SSR markers showed that 38 varieties could be clustered into 2 groups: Sun/Air-Cured and TI tobacco groups, at a relative low level of GS=0.165. And the Sun/Air-Cured group was further classified into 4 subgroups, which was basically according with expected case. The study finally showed that SSR is a stable, reliable and valuable molecular marker system which was more efficient in generating accurate information on genetic background and relationship of tobacco, especially in Sun/Air-Cured germplasms.