Concentration of GPI-Anchored Proteins upon ER Exit in Yeast |
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Authors: | Guillaume A Castillon Reika Watanabe Marcia Taylor Tatjana M E Schwabe Howard Riezman |
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Institution: | Department of Biochemistry, University of Geneva, Sciences II, 30 quai Ernest Ansermet, CH-1211 Geneva, Switzerland |
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Abstract: | Previous biochemical work has revealed two parallel routes of exit from the endoplasmic reticulum (ER) in the yeast Saccharomyces cerevisiae , one seemingly specific for glycosyl-phosphatidylinositol (GPI)-anchored proteins. Using the coat protein II (COPII) mutant sec31-1 , we visualized ER exit sites (ERES) and identified three distinct ERES populations in vivo. One contains glycosylated pro-α-factor, the second contains the GPI-anchored proteins Cwp2p, Ccw14p and Tos6p and the third is enriched with the hexose transporter, Hxt1p. Concentration of GPI-anchored proteins prior to budding requires anchor remodeling, and Hxt1p incorporation into ERES requires the COPII components Sec12p and Sec16p. Additionally, we have found that GPI-anchored protein ER exit is controlled by the p24 family member Emp24p, whereas ER export of most transmembrane proteins requires the Cornichon homologue Erv14p. |
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Keywords: | Bst1p COPII Cwp2p Emp24p ER exit site Erv14p GPI-anchored proteins Hxt1p Per1p |
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