Effects of sugars and growth regulators on <Emphasis Type="Italic">in vitro</Emphasis> growth of <Emphasis Type="Italic">Dactylorhiza</Emphasis> species |
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Authors: | K Wotavová-Novotná H Vejsadová P Kindlmann |
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Institution: | (1) Institute of Systems Biology and Ecology, Academy of Sciences of the Czech Republic, CZ-37005 České Budějovice, Czech Republic;(2) Silva Tarouca Research Institute for Landscape and Ornamental Gardening, CZ-25243 Průhonice, Czech Republic;(3) Faculty of Agriculture, University of South Bohemia, CZ-37005 České Budějovice, Czech Republic |
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Abstract: | The influence of sugars and growth regulators on shoot and root growth of Dactylorhiza species was studied under in vitro conditions. The seedling development was stimulated with the application of glucose and sucrose at concentration of 10 g
dm−3 each. The improvement of shoot growth rate and shoot length was enhanced by cytokinins N
6-(2-isopentenyl)adenine or N
6-benzyladenine and their combination with auxin indolebutyric acid (IBA). The root growth rate and root length of seedlings
increased in the presence of IBA and α-naphthaleneacetic acid. Individual Dactylorhiza species showed statistically significant differences in shoot and root development depending on sugar and growth regulator
combinations. |
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Keywords: | auxins cytokinins glucose in vitro cultivation sucrose terrestrial orchids |
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