Phase separation of cholesterol from phosphatidylserine-cholesterol mixtures in the presence of the local anesthetic tetracaine |
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Authors: | Bach Diana Borochov Nina Wachtel Ellen |
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Affiliation: | a Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel b Holon Academic Institute of Technology, Holon, Israel c Chemical Research Support Unit, Weizmann Institute of Science, Rehovot, Israel |
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Abstract: | ![]() Addition of the local anesthetic tetracaine (TTC) to multilamellar dispersions of natural phosphatidylserine (PS) causes changes in the thermotropic properties of the membrane, which can be detected by differential scanning calorimetry, and in the structure of the membrane as detected by X-ray diffraction. At molar ratio [PS]/[TTC]8.5, the melting temperature of the phospholipid shifts downwards by approximately 2.5 °C. The melting endotherm is broadened; however, there is little change in the enthalpy of melting. In ternary mixtures (PS–TTC–cholesterol), the thermotropic changes are enhanced. At [PS]/[TTC]13, the onset of phase separation of cholesterol crystals from PS in the liquid crystalline state occurs at molar fraction cholesterol (Xchol)0.28, marginally smaller than that found in the absence of the anesthetic. |
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Keywords: | DPPC, 1,2-dipalmitoyl-n-glycero-phosphatidylcholine DOPS, 1,2,-oleyl-n-glycerol-phosphatidylserine DMPS, 1,2-myristoyl-n-glycero-phosphatidylserine PS, natural phosphatidylserine Tm, melting temperature of the acyl chains of the phospholipid Xchol, [chol]/([chol]+[PS]) TTC, tetracaine [PS]/[TTC], molar ratio of PS to tetracaine DSC, differential scanning calorimetry chol, cholesterol lc, liquid crystalline state of the phospholipid |
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