Abstract: | The effects of Cl and pretreatment on 36Clinflux and influx into Characorallina cells were examined. Both treatments reduced 36Clinflux into C. corallina cells in the acid pH range (4.57.0). pretreatment stimulated influx into C. corallina cells, but Cl pretreatment hadno effect. There was a direct inhibitory effect of CI on influx into C. corallina cells, but no apparenteffect on net NO uptake. The time course of 36Cl accumulation into cytoplasmic and vacuolarcompartments during incubation of the cells with showed that significant radioactivity appeared in the vacuolarsap after 30 min. There was a linear increase in the percentageof total 36CI which crossed the tonoplast (c-v). There was noeffect of Cl or pretreatment on accumulation of radioactivity in the vacuole. Thin layer chromatography ofthe vacuolar sap showed that after 2 h only one component waspresent which had an RF which was similar to 36CI. Therate of accumulation of 36C1 in the vacuole could beused to estimate rates of reduction. Key words: Chloride, Chlorate, Chara, Nitrate |