Optimal cell density and multiplicity of infection for the propagation of human rotavirus in monkey kidney cells |
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| Authors: | Kyung Hwa Chang Chang-Ho Park Jong Hwa Park In Sik Chung |
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| Institution: | (1) Departments of Genetic Engineering, Kyung Hee University, Suwon, 449-701, Korea;(2) Chemical Engineering, Kyung Hee University, Suwon, 449-701, Korea |
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| Abstract: | The human rotavirus titer was optimal at an infection cell density of about 4–8 × 104 cells cm–2 in monkey kidney cell cultures. The highest viral titers (3.8 × 107 TCID50 ml–1 and 3.7 × 107 TCID50 ml–1) were obtained at an multiplicity of infection of 0.05 in well plate and T-flask, respectively. |
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| Keywords: | cell density human rotavirus infection multiplicity propagation |
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