Transformation of Claviceps purpurea using a bleomycin resistance gene |
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Authors: | Frank van Engelenburg Ralf Smit Theo Goosen Henk van den Broek Paul Tudzynski |
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Institution: | (1) Institut für Mikrobiologie, Universität Düsseldorf, D-4000 Düsseldorf, Federal Republic of Germany;(2) Department of Genetics, Agricultural University, Dreyenlaan 2, NL-6703 HA Wageningen, The Netherlands;(3) Present address: Botanisches Institut, Universität Münster, Schloßgarten 3, D-4400 Münster, Federal Republic of Germany |
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Abstract: | Summary To develop a DNA-mediated transformation system for Claviceps purpurea a vector was constructed using a bleomycin-resistance gene (bleo
R) fused in frame to the Aspergillus nidulans trp C promoter as a dominant selection marker. The construct was shown to be functional in Aspergillus nidulans and Aspergillus niger and used to transform a wild strain of Claviceps purpurea. Transformats were obtained at low frequencies; they were shown to contain transforming DNA integrated into the chromosomal DNA, probably in multimeric copies and at multiple sites. Combined Southern, Northern and resistance level analysis indicate that the A. nidulans promoter is functional in C. purpurea.Dedicated to Professor Dr. Dr. h. c. K. Esser on the occasion of his 65th birthday |
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