Construction of a genetic linkage map in <Emphasis Type="Italic">Fenneropenaeus chinensis</Emphasis> (Osbeck) using RAPD and SSR markers |
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Authors: | Zhaoning Sun Ping Liu Jian Li Xianhong Meng Xiumei Zhang |
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Institution: | (1) Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China;(2) Chinese Academy of Fishery Sciences, Beijing, 100039, China;(3) Division of Life Sciences and Technology, Ocean University of China, Qingdao, 266003, China |
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Abstract: | The primary genetic linkage maps of Fenneropenaeus
chinensis (Osbeck) were constructed by using the “two-way pseudo-testcross” strategy with RAPD and SSR markers. Parents and F1 progeny
were used as segregating populations. Sixty-one RAPD primers and 20 pairs of SSR primers were screened from 460 RAPD primers
and 44 pairs of SSR primers. These primers were used to analyze the parents and 82 progeny of the mapping family. About 146
primers (128 RAPDs, 18 microsatellites) in the female and 127 primers (109 RAPDs, 18 microsatellites) in the male were segregating
markers. The female linkage map included eight linkage groups, nine triplets and 14 doublets, spanning 1,173 cM with the average
marker density of 11.28 cM, and the observed coverage was 59.36%. The male linkage map included 10 linkage groups, 12 triplets
and seven doublets, spanning 1,144.6 cM with the average marker density of 12.05 cM, and the observed coverage was 62.01%.
The construction of the F. chinensis genetic linkage maps here opened a new prospect for marker-assisted selection program, comparative genomics and quantitative
trait loci (QTL) gene location and cloning. |
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Keywords: | Fenneropenaeus chinensis (Osbeck) Yellow Sea 1 RAPD SSR Genetic linkage map |
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