Transformation of Aspergillus aculeatus using the drug resistance gene of Aspergillus oryzae and the pyrG gene of Aspergillus nidulans |
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| Authors: | Kanamasa Shin Yamaoka Kana Kawaguchi Takashi Sumitani Jun-ichi Arai Motoo |
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| Affiliation: | Graduate School of Agriculture and Biological Sciences, and Research Institute for Advanced Science and Technology, Osaka Prefecture University, Sakai, Japan. |
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| Abstract: | Transformation systems for Aspergillus aculeatus has been developed, based on the use of the pyrithiamine resistance gene of Aspergillus oryzae and the orotidine-5'-monophosphate decarboxylase gene (pyrG) of Aspergillus nidulans. An A. aculeatus mutant which can be transformed effectively by the A. nidulans pyrG gene was isolated as a transformation host. This is the first report of transformation of A. aculeatus. |
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