Agrobacterium tumefaciens-mediated transformation and transgenic-plant regeneration of onion (Allium cepa L.) |
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Authors: | C C Eady R J Weld C E Lister |
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Institution: | (1) New Zealand Institute for Crop & Food Research Ltd, Private Bag 4704, Christchurch, New Zealand e-mail: eadyc@crop.cri.nz Fax: +64-3-3252074, NZ |
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Abstract: | An Agrobacterium tumefaciens-mediated transformation method has been developed for onions (Allium cepa L.) using immature embryos as the explant source. Transgenic plants were recovered from the open-pollinated onion cultivar
Canterbury Longkeeper at a maximum transformation frequency from immature embryos of 2.7%. The method takes between 3–5 months
from explant to primary regenerant entering the glasshouse. Multiple-shoot formation from primary transgenic material made
possible the clonal multiplication of transformants. The binary vector used carried the nptII antibiotic resistance gene and the m-gfp5-ER reporter gene. Transgenic cultures were initially screened for their ability to fluoresce and to grow in the presence of
geneticin (5–25 mg/l). The transgenic nature of individual plants was confirmed by Southern blot analysis.
Received: 12 October 1998 / Revision received: 17 May 1999 Accepted: 14 June 1999 |
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Keywords: | Allium cepa Transformation Regeneration gfp gene Agrobacterium |
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