Crossovers in two German cystic fibrosis families determine probe order for MET, 7C22 and XV-2c/CS.7 |
| |
| Authors: | W Berger J Hein J Gedschold I Bauer A Speer M Farrall R Williamson C Coutelle |
| |
| Institution: | (1) Abteilung für Molekular-und Humangenetik, Zentralinstitut für Molekularbiologie, Akademie der Wissenschaften, DDR-1115 Berlin-Buch, German Democratic Republic;(2) Klinik für Kinderheilkunde, Wilhelm-Pieck-Universität, DDR-2500 Rostock, German Democratic Republic;(3) Abtellung für Biochemie, Wilhelm-Pieck-Universität, DDR-2500 Rostock, German Democratic Republic;(4) Abteilung für Humangenetik, Klinik für Kinderheilkunde, Medizinische Akademie, Leipziger Strasse 44, DDR-3000 Magdeburg, German Democratic Republic;(5) Department of Biochemistry, St. Mary's Hospital Medical School, University of London, Norfolk Place, W2 1PG London, UK |
| |
| Abstract: | Summary We have followed the segregation of the probes pJ3.11, 7C22, pB79a, and MET through cystic fibrosis families in the German Democratic Republic with two affected sibs. Two families with a crossover between MET and the CF phenotype were detected. In one of these families recombination was also observed between the DNA probe 7C22 and CF, and between the markers XV-2c and CF, which suggests that XV-2c, MET and 7C22 are all on the same side of CF. The other MET recombinant family is informative with XV-2c and does not recombine, which excludes the genetic order XC-2c-MET-CF if multiple recombinant events are disregarded. These two families together demonstrate that recombinations may occur in a very small genetic interval, which has important implications for prenatal diagnosis based on data from linked markers. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
