Production of rhoifolin and tiliroside from callus cultures of Chorisia chodatii and Chorisia speciosa |
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| Institution: | 1. Pharmacognosy Department, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt;2. Tissue Culture Unit, Plant Genetic Resources Department, Ecology and Dry Land Agriculture Division, Desert Research Center, 11753 El-Matarya, Cairo, Egypt;3. Pharmacognosy Department, Faculty of Pharmacy, Assiut University, 71515 Assiut, Egypt;1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China;2. Key Laboratory of Medicinal Chemistry for Nature Resource, Ministry of Education, School of Chemical Science and Technology, Yunnan University, Kunming 650091, PRChina;3. Yunnan Institute of Traditional Chinese Medicine and Materia Medica, Kunming, 650223, PR China;1. Centro de Biotecnologia, Departamento de Biotecnologia, Universidade Federal da Paraíba, CEP 58051-970 João Pessoa, PB, Brazil;2. Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba, CEP 58051-900 João Pessoa, PB, Brazil;1. School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China;2. School of Chinese Materia medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;1. Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, P. R. China;2. Reyoung Pharmaceutical Co., Ltd, No. 44 Cultural West Road, Jinan 250012, P. R. China;1. Department of Chemistry and Green-Nano Materials Research Center, Kyungpook National University, 1370 Sankyuk-dong, Taegu 702-701, Republic of Korea;2. Department of Chemistry, Bacha Khan University, Charsadda 24420, KPK, Pakistan |
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| Abstract: | The current work aims to stimulate the production of rhoifolin and tiliroside as two valuable phytochemicals from Chorisia chodatii Hassl. and Chorisia speciosa A. St.-Hil. callus cultures. A comparison between three explants from the in vitro germinated seedlings of both species for callus induction and accumulation of both flavonoids was carried out. Highly efficient calluses were induced from the leaves, stems and roots of C. chodatii seedlings on Gamborg’s B5 (B5) and Murashige and Skoog (MS) media containing 2.0 mg/l β-naphthalene acetic acid (NAA) and 0.5 mg/l 6-benzyladenin (BA) or kinetin (Kn), while those of C. speciosa seedlings efficiently produced calluses on both media supplemented with 0.5 or 1.0 mg/l NAA and 0.5 mg/l BA. Besides, the highest contents of rhoifolin (1.927 mg/g DW) and tiliroside (1.776 mg/g DW) from C. speciosa cultures were obtained from the calluses of seedlings’ roots and stems maintained on B5 medium containing 1.0 mg/l NAA and 0.5 mg/l BA, respectively. On the other hand, the maximum rhoifolin content (0.555 mg/g DW) from C. chodatii cultures was obtained from the calluses of seedlings’ stems grown on B5 medium supplemented with 2.0 mg/l NAA and 0.5 mg/l BA, whereas the highest tiliroside content (0.547 mg/g DW) was provided by the root explants on B5 medium containing 2.0 mg/l NAA and 0.5 mg/l Kn. Both flavonoids were bioaccumulated in greater amounts than the wild and cultivated intact plants, which provides a promising tool for their future commercial production under a controlled environment, independent of climate and soil conditions. |
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| Keywords: | Bombacaceae Callus cultures Flavonoids Rhoifolin Tiliroside |
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