自体软骨细胞修复兔膝关节全层软骨缺损的实验研究

目的：研究自体软骨细胞复合于人脐带Wharton胶支架对兔膝关节全层软骨缺损的修复效果。方法：经自体关节软骨细胞 经体外培养后复合到制备人脐带Wharton 胶取向支架内构建细胞- 支架复合体，选取健康清洁新西兰兔23 只，雌雄不拘，体重 2.5-3.0 kg，取滑车沟中下部制作全层软骨缺损模型后随机分成A、B和C 组。A组(n= 10)：植入自体软骨细胞+人脐带Wharton 胶取向支架复合体；B组(n= 10)：植入单纯人脐带Wharton 胶取向支架；C组(n= 3)：不做任何处理正常兔。分别于术后3 个月和6 个月各处死后取材进行生物力学特性评估检测。结果：压痕实验显示在3 个月时A 和B 组修复区组织刚度分别达到正常软骨的 45.72%和25.25%，且A组刚度明显优于B组，均低于C组( P<0.05)；到6 个月时各自达到正常软骨刚度的69.76%和35.14%，同 样A 组刚度明显优于B 组，均低于C 组( P<0.05)且在同期个各组之间均有显著性差异（F=80.309，P<0.05）。结论：体外培养的自 体软骨细胞与人脐带Wharton 胶复合在体内的微环境作用下修复软骨缺损效果良好，为软骨组织工程提供了一种新支架材料。

Experimental Study of Autologous Chondrocyte Repair of Rabbit Full-thickness Cartilage Defects of the Knee

Objective:To investigate the effect of autologous chondrocytes in human umbilical cord Wharton''s jelly matrix scaffold stents on rabbit knee full thickness cartilage defect repair.Methods:Autologous chondrocyte after in vitro preparation of the composite to build cells from human umbilical cord Wharton''s jelly matrix glue oriented scaffolds - stents complex, choose healthy and clean 23 rabbits, male or female, weighing 2.5-3.0 kg, take the lower pulley groove were randomly divided into A, B and C after making a full thickness cartilage defect model. GroupA (n=10): autologous chondrocyte implantation+human umbilical cord Wharton''s jelly matrix plastic composite scaffold orientation; groupB (n=10): implanted human umbilical cord Wharton''s jelly matrix simple plastic bracket orientation; group C (n=3): no process for normal rabbit. Were everywhere after 3 months and 6 months after the death of biomechanical properties derived assessment test.Results:The indentation experiments are displayed in three months organizing stiffness restoration area A and B , respectively 45.72%and 25.25%of normal cartilage and compare with group A, the stiffness was significantly better than in group B, and both were lower than in groupC (P<0.05); to 6 months when each reached 35.14% and 69.76% of normal cartilage stiffness , compare with group A, the stiffness was significantly better than in group B, and both were lower than in group C (P <0.05) and in the same period of months between the groups was significant difference (F=80.309, P<0.05).Conclusion:Autologous cultured chondrocytes and human umbilical cord Wharton''s jelly matrix scaffold composite repair cartilage defects to good effect in vivo microenvironment for cartilage tissue engineering provides a new scaffold.