H22腹水型肝癌荷瘤小鼠耐受性的性别差异

目的:观察性别对小鼠H22腹水型肝癌生长情况的影响,研究不同性别动物对肝癌耐受性的差异。方法:取30只8周龄昆明鼠,雌雄各半,随机分为四组,实验组每组10只,对照组每组5只,腹腔接种小鼠H22肝癌细胞,建立小鼠H22腹水型肝癌模型。每天测量小鼠体重并记录生存时间,直至实验组小鼠全部死亡,比较性别因素对小鼠H22腹水型肝癌的生存期是否存在差异。结果:小鼠接种瘤细胞后,逐渐产生腹水,体重增加。雌性小鼠的体重增加比雄性小鼠显著,P=0.049。雄性小鼠生存后期体重出现下降,呈明显恶液质状态。雌性小鼠的体重、腹水增加虽然较雄性动物明显,但生存期却并不少于雄性鼠,反而比雄性小鼠略长,P=0.1567。结论:性别对小鼠H22腹水型肝癌的生长有一定的差异,雌性小鼠的耐受性优于雄性小鼠。

Effect of Gender on the Survival of H22 Liver Cancer Bearing Mice

Objective:To observe the effect of gender on the survival of H22 liver cancer bearing mice.Methods:Thirty 8-week old Kunming mice, half males and half females, were randomly divided into four groups, i.e., male tumor bearing group with 10 animals, male control group with 5 animals, female tumor bearing group with 10 animals, and female control group with 5 animals. Survival analysis was conducted by Kaplan-Meier survival function and the log-rank test.Results:After inoculated with H22 cells, the animals showed sign of ascites in a few days, and body weight grow faster than control animals due to the accumulation of ascites. At the end stage of life, the male mice demonstrated a significant body weight decrease due to cachexia. The median survival of female mice was 20.5 days, slightly longer than that of male animals (19.5 days). The statistical analysis was not significant though (P=0.1567).Conclusion:Male animals are less tolerant to the liver cancer than female mice.