肾动脉射频消融对兔压力负荷性高血压的作用研究

目的：建立新西兰兔的高血压模型和观察肾动脉射频消融（Renal Sympathetic Denervation，RSD）对高血压的影响。方法：通 过腹主动脉内径缩窄50%~60%建立兔高血压模型，建模后分为假手术对照组、对照组+ 射频组、高血压组、高血压+ 射频组4 组，建模2个月后使用临床常用的射频消融导管经腹进行双侧肾动脉外膜消融去除肾交感神经，术后继续饲养2 个月，通过颈动 脉插管测定血压数值变化。结果：通过腹主动脉缩窄术成功建立新西兰兔压力负荷高血压模型。RSD 术后2月，新西兰兔的心率 无明显区别（P>0.05），高血压组的血压仍然明显高于对照组（P<0.05），然而，高血压+射频组的血压低于高血压组（P<0.05）；同时 对照+射频组与对照组的血压无明显差异(P>0.05)。结论：通过腹主动脉缩窄术可以稳定地建立新西兰兔压力负荷高血压模型， 经RSD 后血压明显下降，且这种降压作用只对高血压有作用。我们首次从动物水平验证RSD可以降低压力负荷导致的血压升 高。

Effects of Renal Sympathetic Denervation on Pressure-overload Hypertension in Rabbits

Objective:To observe the effects of Renal Sympathetic Denervation (RSD) on blood pressure with pressure-overload animal model.Methods:The rabbit model of pressure overload hypertension was established by 50%~ 60%coarctation of the abdominal aorta based on previous reports. After two months, the abdominal RSD was carried out with radiofrequency ablation catheter. Two months after RSD, the blood pressure value was measured by carotid artery intubation.Results:The rabbit model of hypertension was successfully established by abdominal aortic constriction. Two months after RSD, the heart rate of rabbits in four groups had no significant difference (P>0.05). The blood pressure in hypertension group remained significantly higher than that of control group (P<0. 05). However, blood pressure in hypertension+ablation group was lower than that of hypertension group (P<0.05); while there was no significant difference between control+ablation group and control group (P>0.05).Conclusion:The pressure-overload hypertension model of New Zealand rabbits can be stably established by abdominal aortic coarctation. RSD can reduce blood pressure caused by pressure-overload, which is good to cardiac load for a long time.