EBV阳性胃癌细胞系与阴性胃癌细胞系全基因组表达谱差异比较

目的：探讨EB病毒阳性胃癌细胞系与EB 病毒阴性胃癌细胞系全基因组表达谱的差异。方法：利用Agilent 人类全基因组 表达谱芯片技术在3 种EB病毒阳性胃癌细胞系和3 种EB 病毒阴性胃癌细胞系中筛选EB病毒相关胃癌肿瘤相关基因。选取6 条差异表达基因，应用实时荧光定量PCR验证芯片结果的可靠性。结果：聚类热图及矩阵图分析显示，EB病毒阳性胃癌细胞系与 阴性胃癌细胞系之间表达谱存在明显差异。差异表达基因涉及多种生物学过程。选取的6 条差异基因进行验证，其表达趋势与芯 片结果一致。结论：感染EB病毒可能会改变肿瘤相关基因的表达，进而在EB病毒相关胃癌的发生、发展及预后中发挥作用。筛 选出的肿瘤相关基因涉及多种生物学过程，提示多基因及相关基因的变异参与了EB病毒相关胃癌的形成。

Study on Gene Expression Profiles Discrepancy of EBV Positive and EBV Negative Gastric Carcinoma Cell Lines

Objective:To compare the whole gene expression profile between EBV positive gastric carcinoma cell lines and EBV negative ones.Methods:Agilent Human Whole Genome Expression Profiling chips were used to detect and analyze the whole gene expression profile of EBVaGC in 3 EBV positive and 3 negative gastric carcinoma cell lines. Six candidate genes were chosen for real-time PCR validation.Results:Heat map and matrix plot analysis found distinctly different expression profile between EBV positive gastric carcinoma cell lines and negative gastric carcinoma cell lines. These tumor associated genes involve many biological process. The expression trend of 6 genes for further verification was consistent with the microarray.Conclusion:Our study suggests that EBV infection may change the expression of tumor associated genes, which may play roles in the formation, development and prognosis of EBVaGC. The differentially-expressed genes involve many biological processes, suggesting that many genes and their mutations play roles in the formation of EBVaGC.