| 生物催化法合成6-氰基-（3R，5R）-二羟基己酸叔丁酯 |
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| 引用本文： | 肖黎,王亚军,曹政,柳志强,郑裕国.生物催化法合成6-氰基-（3R，5R）-二羟基己酸叔丁酯[J].生物加工过程,2013,11(1):29-34. |
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| 作者姓名： | 肖黎 王亚军 曹政 柳志强 郑裕国 |
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| 作者单位： | 浙江工业大学生物工程研究所生物转化与生物净化教育部工程研究中心 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)资助(2011CB710800) |
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| 摘 要： | 利用E.coli BL21／pCDFDuet-gdh—cr-X共表达全细胞催化6-氰基-（5R）-羟基-3-羰基己酸叔丁酯不对称还原合成6-氰基-（3R，5R）-二羟基已酸叔丁酯。结果表明：在菌体用量4．85g/L、葡萄糖与底物质量浓度比为1：1、温度28℃、pH7．0条件下，80．0g／L6-氰基-（5R）-羟基-3-羰基己酸叔丁酯生物还原2h后，底物转化率可达99．0％，产物d.e．值大于99．5％。在考察范围内，NADP^＋用量对催化效率无显著作用。
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| 关 键 词： | 6-氰基-（3R，5R）-二羟基己酸叔丁酯 羰基还原酶 葡萄糖脱氢酶 共表达 |
Development of biocatalytic process for t-butyl 6-cyano-(3R,5R)-dihydroxylhexanoate |
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| XIAO Li,WANG Yajun,CAO Zheng,LIU Zhiqiang,ZHENG Yuguo.Development of biocatalytic process for t-butyl 6-cyano-(3R,5R)-dihydroxylhexanoate[J].Chinese Journal of Bioprocess Engineering,2013,11(1):29-34. |
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| Authors: | XIAO Li WANG Yajun CAO Zheng LIU Zhiqiang ZHENG Yuguo |
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| Institution: | (Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education,Institute of Bioengineering, Zhejiang University of Technology,Hangzhou 310014,China) |
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| Abstract: | A co-expression strain, E. coli BL21/pCDFDuet-gdh-cr-X, carrying cr-X and gdh genes, displaying good diastereoselective R-ketoreductase activity towards t-butyl 6-cyano-（ 5R）-hydroxy-3- oxohexanoate. Effects of temperature, pH cell loading and concentrations of glucose, substrate, NADP ＋ on biotrans-formation efficiency were investigated. Results indicated that under the optimal conditions of 28 ℃, pH 7.0, mass ratio of glucose to substrate at 1：1 （w/w） ,80.0 g/L t-butyl 6-cyano-（5R） -hydroxy- 3-oxohexanoate was completely converted to t-butyl 6-cyano-（ 3R, 5R）-dihydroxylhexanoate in 2 h with d.e. over 99.5%. In addition, no significant impact of NADP ＋ concentration was observed in the test range on the asymmetric reduction of t-butyl 6-cyano-（5R）-hydroxyl-3-oxohexanoate. |
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| Keywords: | t-butyl 6-cyano-（ 3R 5R） -dihydroxylhexanoate carbonyl reductase glucose dehydragenase co-expression |
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