| Construction and packaging of pseudotype retrovirus containing human N—ras cDNA antisense sequence and its biological effects on human hepatoma cells |
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| 引用本文： | JIALIBIN WANGXIANG 等.Construction and packaging of pseudotype retrovirus containing human N—ras cDNA antisense sequence and its biological effects on human hepatoma cells[J].细胞研究,1990,1(2):131-139. |
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| 作者姓名： | JIALIBIN WANGXIANG |
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| 作者单位： | [1]NationalResearchCenterforOrcogenesandRelatedGenes,ShanghaiCancerInstitute,China [2]NationalResearchCente,ShanghaiCancerInstitute,China |
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| 摘 要： | N-ras is one of the transforming genes in human hepatic cancer cells.It has been found that N-ras was overexpressed at the mRNA and protein level in hepatoma cells.In order to explore the biological roles of N-ras in human hepatic carcinogenesis and the potential application in control of cancer cell growth,a preudotype retrovirus containing antisense sequence of human N-ras was constructed and packaged.A recombinant retrovirus vector containing antisense or sense sequences of N-ras cDNA was constructed by pZIP-NeoSV(X)1.The pseudotype virus was packaged ang rescued by transfection and infection in PA317 and ψ 2 helper cells.It has been demonstrated that the pseudotype retrovirus containing antisense N-ras sequence did inhibit the growth of human PLC/PRF/5 hepatoma cells accompanied with inhibition of p21 expression,while the retrovirus containing sense sequence had none.The pseudotype virus had no effect on human diploid fibroblasts.
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| 关 键 词： | 人肝癌细胞 逆转康病毒 人N－ras cDNA 反义序列 构建 包装 生物学作用 |
Construction and packaging of pseudotype retrovirus containing human N-ras cDNA antisense sequence and its biological effects on human hepatoma cells |
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| JIA LIBIN,WANG XIANG,XU XITJLAN,MA JIQING,YANG JIN-YING,LIU OHAOTING AND GU JIANREN
National Research Center fcr Orcogenes and Relaied Genes,Shanghai Cancer Institute,China.Construction and packaging of pseudotype retrovirus containing human N-ras cDNA antisense sequence and its biological effects on human hepatoma cells[J].Cell Research,1990,1(2):131-139. |
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| Authors: | JIA LIBIN WANG XIANG XU XITJLAN MA JIQING YANG JIN-YING LIU OHAOTING AND GU JIANREN
National Research Center fcr Orcogenes and Relaied Genes Shanghai Cancer Institute China |
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| Abstract: | |
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| Keywords: | N-ras cDNA antisense RNA retroviral vector hepatic cell |
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