毛喉鞘蕊花提取物对大鼠哮喘模型炎症介质的影响及机制研究

为探讨毛喉鞘蕊花提取物(Coleus Forskohlii extract,CFE)对哮喘的治疗作用及机制,采用卵清蛋白(ovalbumin,OVA)结合佐剂氢氧化铝建立大鼠哮喘模型,SPF级SD雄性大鼠60只,随机分为5组,即空白组、模型组、地塞米松组、CFE高剂量组(12.8g/kg)和CFE低剂量组(6.4g/kg),分别用生理盐水、地塞米松及CFE每天灌胃1次,连续14天,灌胃容积为1mL/100g。通过HE染色观察肺组织病理学形态学变化;ELISA法测定大鼠血清(serum)及肺泡灌洗液(BALF)中IFN-γ、IL-4和IL-17A含量变化;Western blot检测MAPK通路相关蛋白的表达变化。HE结果显示,与模型组相比,CFE高、低剂量组能显著减轻OVA刺激后大鼠肺组织病理性损伤,气道轮廓清晰,上皮细胞脱落明显减少;ELISA结果显示,与模型组相比,CFE高、低剂量组大鼠血清及BALF中IL-4、IL-17A的含量明显下降,IFN-γ的含量明显上升(P<0.05或P<0.01);WB结果显示,CFE高剂量组可显著降低大鼠肺组织p-ERK、p-JNK及p-p38蛋白表达(P<0.05或P<0.01)。综上,毛喉鞘蕊花提取物可有效缓解OVA诱导的大鼠哮喘气道炎症反应,其机制与调节MAPK信号传导通路有关。

Effect of Coleus Forskohlii extract on the inflammatory mediators in rats asthmatic model and mechanism

To explore the therapeutic effect and mechanism of Coleus Forskohlii extract (CFE) on asthma,the rat asthma model was established by ovalbumin (OVA) combined with adjuvant aluminum hydroxide.Sixty SPF-SD male rats were divided into blank group,model group,dexamethasone group,CFE high-dose group (12.8 g/kg) and CFE low-dose group (6.4 g/kg),normal saline,dexamethasone and CFE were administered to the stomach once a day for 14 days consecutively in groups,with a gavage volume of 1 mL/100 g.HE staining was used to observe the histopathological changes of lung;ELISA was used to detect the expression of IFN-γ,IL-4 and IL-17A;Western blot assay was used to detect expression of related MAPK pathway protein.Compared with the model group,HE results showed that the high and low dose groups of CFE could significantly reduce the pathological damage of the lung tissue after OVA stimulation,with clear airway contour and significantly reduced epithelial cell shedding;ELISA results showed that the levels of IL-4 and IL-17A in serum and BALF of rats in the high and low dose CFE groups decreased,while the level of IFN-γ increased,with statistically significant differences ( P < 0.05 or P < 0.01);WB results showed that the high dose group of CFE reduced significantly the expression of p-ERK、p-JNK and p-p38 proteins in rats lung tissues ( P < 0.05 or P < 0.01).To sum up,Coleus Forskohlii extract can effectively alleviate the airway inflammation induced by OVA in rats with asthma,and the mechanism is related to the regulation of MAPK signaling pathway.