大胡蜂蜂毒中多肽和蛋白质结构和功能的多样性

为证明大胡蜂Vespa magnifica(Smith)蜂毒具有较大的药用开发价值,本研究采用超高效液相色谱-质谱和电泳技术对其多肽和蛋白质的分布进行分析,发现其蛋白质的相对分子质量主要分布在17~45kDa范围内。蜂毒多肽类物质的相对分子质量呈"单峰"式分布,61%在500~3000Da范围内,为大胡蜂蜂毒中多肽含量最为丰富的部分。通过牛津杯法对蜂毒的抑菌活性进行研究,且以HepG2人肝癌细胞及B16黑色素瘤细胞为研究对象,用MTT法检测蜂毒的细胞毒性活性,证明其具有良好的抑菌作用和细胞毒活性,其结果与已报道的其他蜂类既有相似性又存在具体差异,展示了大胡蜂蜂毒的分子多样性,为后续该毒素的物质基础研究及药用价值开发提供参考。

Determination of peptide and protein diversity in venom of Vespa magnifica(Smith)on structure and function

In order to prove that Vespa magnifica (Smith) venom has great medicinal purposes development value.In this study,the distribution of molecular mass of peptides and proteins was analyzed by Ultra Performance Liquid Chromatography-Electrospray-Quadrupole-Time of Flight Mass Spectrometry (UPLC-ESI-Q-TOF-MS) and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE).The relative molecular mass of the proteins is mainly distributed in the range of 17 to 45 kDa.The peptides present a unimodal distribution and 61% of them are in the range of 500-3 000 Da,which is the most abundant part of the crude venom of V.magnifica.The antibacterial activity of the venom was studied by Oxford Cup method.HepG2 human hepatoma cells and B16 melanoma cells were used as the research object to evaluate the cytotoxic activity of the venom by MTT assay,which proved the potential antibacterial activity and cytotoxic activity.The results have similarities and specific differences with other reported bees and wasp,showing the molecular diversity of the venom and providing reference for the subsequent material research and medicinal value development of the venom.