HPLC同时测定大叶冬青叶中10种多酚成分的含量及抗氧化活性研究

建立HPLC同时测定大叶冬青叶中新绿原酸、绿原酸、隐绿原酸、咖啡酸、对羟基肉桂酸、芦丁、异槲皮苷、异绿原酸B、异绿原酸A和异绿原酸C 10种多酚类化合物的定量分析方法,研究不同产地及不同采收时期大叶冬青叶中10种多酚类化合物的含量差异及其抗氧化活性。采用HPLC测定大叶冬青叶50%甲醇提取液中10种多酚类成分的含量,并选用DPPH法对其抗氧化活性进行初步探索。结果表明,10种多酚类化合物分离效果较好,标准曲线在检测范围内具有良好的线性(r>0.999 5),平均加样回收率在96.58%～112.03%,RSD<4%(n=6)。大叶冬青叶50%甲醇提取液抗氧活性良好。本实验建立的方法快速、准确、重复性好,可用于同时测定大叶冬青叶中新绿原酸、绿原酸、隐绿原酸、咖啡酸、对羟基肉桂酸、芦丁、异槲皮苷、异绿原酸B、异绿原酸A、异绿原酸C 10种成分的含量;不同产地、不同采收时期大叶冬青叶中10种多酚类化合物含量存在一定差异;不同来源大叶冬青叶50%甲醇提取液抗氧化能力具有明显差异。

Determination of 10 polyphenol components by HPLC and antioxidant activity in the leaves of Ilex latifolia

This research established an HPLC method for determination of ten kinds of polyphenol including the neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,p-hydroxycinnamic acid,rutin,isoquercitrin,isochlorogenic acid B,isochlorogenic acid A and isochlorogenic acid C in the leaves of Ilex latifolia ,and investigated the content difference and the antioxidant activity.Determination of 10 polyphenols in 50% methanol extract from the leaves of I.latifolia by HPLC and preliminary study on its antioxidant activity by DPPH.The results showed that Ten compounds were well separated with good linear correlations ( r >0.999 5).The average recoveries of ten compounds were 96.58%-112.03% and the RSD were less than 4%( n =6).The 50% methanol extract from the leaves of I.latifolia has good antioxidant activity.The HPLC method was rapid,accurate and stable,which could be used to detect simultaneously neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,p-hydroxycinnamic acid,rutin,isoquercitrin,isochlorogenic acid B,isochlorogenic acid A and isochlorogenic acid C in the leaves of I.latifolia. There are certain differences in the contents of ten compounds between the samples from different producing areas and harvest times.The antioxidant capacity of 50% methanol extracts from the leaves of I.latifolia from different sources is significantly different.