基于网络药理学的谷糠结合态多酚抗乳腺癌机制研究

本研究旨在探讨谷糠结合态多酚(bound phenol of inner shell,BPIS)发挥抗乳腺癌细胞活性的作用机制。首先采用细胞计数法检测BPIS对乳腺癌细胞以及正常乳腺细胞活性的影响;然后综合运用SEA、SIB以及GeneCards等数据库获得BPIS和乳腺癌的相关靶点,并分析活性成分与作用靶点的互作网络以及通路。本研究筛选得到BPIS抗乳腺癌相关靶点39个,主要涉及糖脂代谢和细胞自噬等生物过程以及MAPK、PI3K/AKT、FoxO等多条信号通,表明BPIS抗乳腺癌是多成分、多靶点、多通路协同作用的过程,而与细胞死亡相关的细胞自噬很可能在BPIS抑制乳腺癌过程中发挥主要作用。

The mechanism of BPIS against breast cancer based on network pharmacology

The aim of this study was to investigate the mechanism of BPIS (bound phenol of inner shell) against breast cancer cell. Firstly, the effect of BPIS on the activity of breast cancer cells and normal breast cells was detected by cell counting. Secondly, the SEA, SIB and GeneCards databases were used to predict and screen the targets of BPIS on breast cancer. Then, the String database, Cytoscape software and DAVID database were used to construct the active components-targets network and analyse pathway. After these studies,39 targets were screened, which involved in biological processes such as Glycolipid metabolism, autophagy and so on, and signaling pathways such as MAPK, PI3K/AKT, FoxO and so on. These results indicated that BPIS anti-breast cancer is a multi-component, multi-target, multi-signal synergistic process, and autophagy associated with cell death is likely to play a major role in BPIS inhibition of breast cancer.