川白芷抑菌活性及对铜绿假单胞菌群体感应的抑制作用

为寻找更加有效的抑菌杀菌药物,本研究利用琼脂平板打孔法和倍比稀释法评价川白芷不同溶剂萃取物对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌以及肺炎克雷伯氏菌的抗菌活性;特别是通过对铜绿假单胞菌毒力表型的影响研究考察该药物对病菌群体感应是否具有抑制作用。研究结果表明:川白芷提取液对四种细菌均有不同程度的抑制效果,且随提取物浓度增大抑制效果增强;进一步分离发现,三氯甲烷、乙酸乙酯和正丁醇提取物对四种细菌均有一定的抑制效果,其中乙酸乙酯萃取物的抑菌效果最佳;对铜绿假单胞菌的四种毒力表型的影响研究结果表明川白芷具有抑制铜绿假单胞菌群体感应的能力。试验表明川白芷的活性成分可以作为一种新型的群体感应抑制剂,其具有抑制多种细菌能力的同时不易产生耐药性,表明白芷这一传统中草药在现代医疗中具有较好的应用潜力。

Bacteriostatic activity of Angelicae dahuricae and their inhibitory effect of Pseudomonas aeruginosa on the quorum sensing

In order to find drugs with more effective bacteriostatic and bactericidal action,this study evaluated the antibacterial activity of Angelicae dahuricae on Staphylococcus aureus,Escherichia coli,Pseudomonas aeruginosa and Klebsiella pneumoniae by using agar plate perforation method and double dilution method.In particular,the influence of the drug on the quorum sensing of pathogenic bacteria was tested by investigating the virulence phenotype of Pseudomonas aeruginosa.The results showed that the extracts of Angelicae dahuricae had different inhibitory effects on four kinds of bacteria,and the inhibitory effect increased with the increase of extract concentration.Further separation found that trichloromethane,ethyl acetate and N-butanol extract had a certain inhibitory effect on four kinds of bacteria,of which the ethyl acetate extract was the best.The tests of four virulence phenotypes of Pseudomonas aeruginosa showed that Angelicae Dahuricae had the ability to inhibit the quorum sensing of this bacteria.To summarise,this research showed that the active components of Angelicae dahuricae could be used as a new type of Quorum Sensing inhibitor,which had the ability to inhibit many kinds of bacteria and would not produce drug resistance at the same time,it means that this traditional Chinese herb has a cheerful prospect in modern medicine.