| 茯神挥发性成分及其生物活性研究 |
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| 引用本文： | 王军民,张加研,雷福厚,陈凯,严毅.茯神挥发性成分及其生物活性研究[J].天然产物研究与开发,2019(8):1402-1407,1356. |
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| 作者姓名： | 王军民 张加研 雷福厚 陈凯 严毅 |
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| 作者单位： | 西南林业大学西南山地森林资源保育与利用教育部重点实验室;西南林业大学化学工程学院;广西民族大学广西林产化学与工程重点实验室;昆明市海口林场 |
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| 基金项目： | 云南省科技计划(2016FD045);农业联合面上项目(2017FG001(-047));广西林产化学重点实验室开放课题(GXFC14-12);昆明市科技计划(2016-1-S-03064) |
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| 摘 要： | 为研究茯神挥发性成分含量、构成及其生物活性,本实验采用二氧化碳超临界的方法提取茯神低极性成分,以提取率作为响应值,在单因素试验的基础上采用响应曲面法考察提取压力、温度、二氧化碳流速对提取效果的影响,优化提取工艺。运用气相色谱-质谱联用仪(GC-MS)对其主要化学成分进行鉴定,并采用生长速率法测定了挥发油对5种真菌的生物活性。得到提取模型极值点,即提取压力27.26MPa、提取温度55.97℃、提取流速10.68L/min时,提取率达到最大,提取率预测值为1.66mg/g。通过NIST14质谱库检索,鉴定了其中17个主要化合物,运用峰面积归一法确定各个组分的含量,占挥发油总量的84.5%。抗菌实验表明茯神挥发油对采绒革盖菌菌株有低浓度促进高浓度抑制的活性。
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| 关 键 词： | 茯神 挥发性成分 提取工艺 生物活性 |
The volatile components from Poria with hostwood and their bioactivities |
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| WANG Jun-min,ZHANG Jia-yan,LEI Fu-hou,CHEN Kai,YAN Yi.The volatile components from Poria with hostwood and their bioactivities[J].Natural Product Research and Development,2019(8):1402-1407,1356. |
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| Authors: | WANG Jun-min ZHANG Jia-yan LEI Fu-hou CHEN Kai YAN Yi |
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| Institution: | (Key Laboratory for Forest Resources Conservation and Utilization in Southwest Mountainous of China,Southwest Forestry University;School of Chemical Engineering,Southwest Forestry University,Kunming 650224 ,China;Guangxi Key Llaboratory of Chemistry and Engineer of Forest Products,Guangxi University for Nationalities,Nanning 530006 ,China;The Forest Farms from Haikou in Kunming,Kunming 650114 ,China) |
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| Abstract: | In order to study the content,composition and biological activities of volatile components from Poria with hostwood,the volatile components weas extracted by the method of supercritical carbon dioxide.The extraction technology was optimized by response surface method based on the single factor test,with the extraction rate of volatile as an index and three factors investigated which contained pressure,temperature and carbon dioxide flow rate.The chemical compositions were analyzed by gas chromatography-mass spectrometry (GC-MS).Also the antibacterial activities of the volatile to 5 strains of fungi were studied by the method of plate growth rate.As a result,the best extraction conditions were as follows:extraction pressure of27.26 MPa,extraction temperature of 55.97 ℃ and carbon dioxide flow rate of10.68 L/min.Totally17 main compounds were identified by NIST14 mass spectral library retrieval,and the relative content was determined with the peak area normalization method,which was up to 84.5% of the volatile oil.The antibacterial experiment showed that the volatile oil had bioactivities to Coriolus versicolor . |
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| Keywords: | Poria with hostwood volatile constituent extraction process bioactivities |
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